ASYMMETRICALLY DISTRIBUTED PAR-3 PROTEIN CONTRIBUTES TO CELL POLARITY AND SPINDLE ALIGNMENT IN EARLY C-ELEGANS EMBRYOS

作者：ETEMADMOGHADAM B^*; GUO S; KEMPHUES KJ

来源：Cell, 1995, 83(5): 743-752.

DOI：10.1016/0092-8674(95)90187-6

The par-3 gene is required for establishing polarity in early C. elegans embryos. Embryos from par-3 homozygous mothers show defects in segregation of cytoplasmic determinants and in positioning of the early cleavage spindles. We report here that the PAR-3 protein is asymmetrically distributed at the periphery of the zygote and asymmetrically dividing blastomeres of the germline lineage. The PAR-3 distribution is roughly the reciprocal of PAR-1, another protein required-for establishing embryonic polarity in C. elegans. Analysis of the distribution of PAR-3 and PAR-1 in other par mutants reveals that par-2 activity is required for proper localization of PAR-3 and that PAR-3 is required for proper localization of PAR-1. In addition, the distribution of the PAR-3 protein correlates with differences in cleavage spindle orientation and suggests a mechanism by which PAR-3 contributes to control of cleavage pattern.

• 出版日期1995-12-1