Usefulness of a genotypic resistance test using dried blood spot specimens in African HIV-infected children with virological failure according to the 2010-revised WHO criteria

作者：Charpentier Charlotte^*; Gody Jean Chrysostome; Tisserand Pascaline; Matta Mathieu; Fournier Julien; Mbitikon Olivia; Belec Laurent

来源：Archives of Virology, 2011, 156(9): 1603-1606.

DOI：10.1007/s00705-011-0997-9

• Antiretroviral drug resistance
• Central African Republic
• HIV-1 non-B subtypes
• Dried blood spot
• Children

We compared paired plasma and dried blood spot (DBS) samples from 54 HIV-1-treated children living in Bangui, Central African Republic, for antiretroviral-resistance-associated mutations. All children displayed virological failure (HIV-1 RNA > 3.70 log(10)copies/ml). Testing for resistance genotype was carried out in a reference laboratory in Paris, France. A successful test result was obtained in 54 (100%) plasmas and 25 DBSs (46%). Among the 732 resistance-associated mutations analyzed, 718 were identical, leading to a high concordance rate of 98.1%. Genotypic resistance tests on DBS samples were found to be highly feasible and accurate in a foreign reference laboratory, but with additional costs for shipping and decreased sensitivity.

• 出版日期2011-9