Mineralization behavior with mesenchymal stromal cells in a biomimetic hyaluronic acid-based scaffold

作者：Manferdini Cristina; Guarino Vincenzo; Zini Nicoletta; Raucci Maria Grazia; Ferrari Andrea; Grassi Francesco; Gabusi Elena; Squarzoni Stefano; Facchini Andrea; Ambrosio Luigi; Lisignoli Gina^*

来源：Biomaterials, 2010, 31(14): 3986-3996.

DOI：10.1016/j.biomaterials.2010.01.148

• Biomimetic material
• Hyaluronic acid
• SBF
• Fibroblast growth factor
• Osteogenesis

A biomimetic hyaluronic acid (HA)-based polymer scaffold was analysed in vitro for its characteristics and potential to support mineralization as carrier-vehicle. Biomimetic apatite crystal nucleation on the scaffold surface was obtained by a fine control of the pH level that increased ionic solubility thus controlling apatite formation kinetic. Different concentrations of human mesenchymal stromal cells (h-MSCs) were seeded on the scaffold, osteogenesis was induced in the presence or absence of fibroblast growth factor -2 and mineralization was analysed at different time points. We found that only at the highest h-MSCs concentration tested, the cells were uniformly distributed inside and outside the scaffold and proliferation started to decrease from day 7. Electron microscopy analysis evidenced that h-MSCs produced extracellular matrix but did not establish a direct contact with the scaffold. We found mineralized calcium-positive areas mainly present along the backbone of the scaffold starting from day 21 and increasing at day 35. FGF-2 treatment did not accelerate or increase mineralization. Non-biomimetic HA-based control scaffold showed immature mineralized areas only at day 35. Our data demonstrate that the biomimetic treatment of an HA-based scaffold promotes a faster mineralization process suggesting its possible use in clinics as a support for improving bone repair.

• 出版日期2010-5