Application of massively parallel pyrosequencing to the discovery of non-dinucleotide microsatellites: tetra- and pentanucleotide repeat markers for the Pacific abalone (Haliotis discus hannai)

作者：Sekino Masashi^*; Shigenobu Yuya; Nakamura Yoji; Sugaya Takuma; Hara Motoyuki

来源：Conservation Genetics Resources, 2012, 4(4): 939-941.

DOI：10.1007/s12686-012-9678-6

• Next-generation sequencing
• Short tandem repeat
• Simple sequence repeat

The accelerated progress of ultra high-throughput DNA sequencing technologies has facilitated the process of identifying microsatellites in the genomes, attenuating laborious experimental procedures involved in traditional approaches. We applied a massively parallel pyrosequencing technique to the development of tetra- and pentanucleotide microsatellite markers for the Pacific abalone Haliotis discus hannai.

• 出版日期2012-12