The use of CD138 positively selected marrow samples increases the applicability of minimal residual disease assessment by PCR in patients with multiple myeloma

作者：Puig Noemi; Sarasquete Maria E; Alcoceba Miguel; Balanzategui Ana; Chillon Maria C; Sebastian Elena; Marin Luis A; Gonzalez Diaz Marcos; San Miguel Jesus F; Garcia Sanz Ramon

来源：Annals of Hematology, 2013, 92(1): 97-100.

DOI：10.1007/s00277-012-1566-3

• Multiple myeloma
• Minimal residual disease
• Real-time quantitative PCR (RQ-PCR)
• CD138+selection
• Applicability

We have evaluated the use of CD138+ positively selected bone marrow samples to identify a molecular target for minimal residual disease assessment by polymerase chain reaction (PCR) in 25 untreated patients with multiple myeloma. A fraction of each sample was used for CD138+ selection, and the rest served as a reference control. VDJH, DJH, and Kde gene rearrangements were tested for amplification according to the BIOMED-2 Concerted Action. PCR products were directly sequenced in an automated ABI 3130 DNA sequencer using Big-Dye terminators. Within the CD138+ selected group, VDJH rearrangements were detected in all cases (100 %), DJH in 16 (64 %), and Kde in 18 (72 %) cases; whereas in the control samples, VDJH, DJH, and Kde rearrangements were detected in 19 (76 %), 11 (44 %), and 12 (48 %) cases, respectively. After sequencing, 24 (96 %) cases within the CD138+ group had a PCR target for MRD detection compared with 15 (60 %) cases in the control group. We conclude that the use of CD138+ positively selected bone marrow samples increases the applicability of minimal residual disease studies by PCR in patients with multiple myeloma.

• 出版日期2013-1