Amperometric glucose sensor based on enhanced catalytic reduction of oxygen using glucose oxidase adsorbed onto core-shell Fe3O4@silica@Au magnetic nanoparticles

作者：Wang, Ai-Jun; Li, Yong-Fang; Li, Zhong-Hua; Feng, Jiu-Ju^*; Sun, Yan-Li; Chen, Jian-Rong

来源：Materials Science & Engineering C-Materials for Biological Applications, 2012, 32(6): 1640-1647.

DOI：10.1016/j.msec.2012.04.055

• Fe3O4@silica@Au nanoparticles
• Glucose biosensor
• Electron transfer

Monodisperse Fe3O4 magnetic nanoparticles (NPs) were prepared under facile solvothermal conditions and successively functionalized with silica and Au to form core/shell Fe3O4@silica@Au NPs. Furthermore, the samples were used as matrix to construct a glucose sensor based on glucose oxidase (GOD). The immobilized GOD retained its bioactivity with high protein load of 3.92 x 10(-9) mol.cm(-2), and exhibited a surface-controlled quasi-reversible redox reaction, with a fast heterogeneous electron transfer rate of 7.98 +/- 0.6 s(-1). The glucose biosensor showed a broad linear range up to 3.97 mM with high sensitivity of 62.45 mu A.mM(-1) cm(-2) and fast response (less than 5 s).

• 出版日期2012-8-1
• 单位河南科技学院; 河南师范大学; 浙江师范大学