Detection of S-nitrosothiols in biological fluids: A comparison among the most widely applied methodologies

作者：Giustarini Daniela; Milzani Aldo; Dalle Donne Isabella; Rossi Ranieri^*

来源：Journal of Chromatography B-Analytical Technologies in the Biomedical and Life Sciences, 2007, 851(1-2): 124-139.

DOI：10.1016/j.jchromb.2006.09.031

• reviews
• nitric oxide
• S-nitrosoalbumin
• S-nitrosohemoglobin
• S-transnitrosation reactions

Many different methodologies have been applied for the detection of S-nitrosothiols (RSNOs) in human biological fluids. One unsatisfactory outcome of the last 14 years of research focused on this issue is that a general consensus on reference values for physiological RSNO concentration in human blood is still missing. Consequently, both RS NO physiological function and their role in disease have not yet been clarified. Here, a summary of the values measured for RSNOs in erythrocytes, plasma, and other biological fluids is provided, together with a critical review of the most widely used analytical methods. Furthermore, some possible methodological drawbacks, responsible for the highlighted discrepancies, are evidenced.

• 出版日期2007-5-15