DETECTION AND TYPING OF GENITAL PAPILLOMAVIRUSES IN MEN WITH A SINGLE POLYMERASE CHAIN-REACTION AND TYPE-SPECIFIC DNA PROBES

作者：MAZZATENTA C; ANDREASSI L; BIAGIOLI M; RICCI S; RATTI G

来源：Journal of the American Academy of Dermatology, 1993, 28(5): 704-710.

Background: Human papillomavirus (HPV) infection of the genitalia can produce both visible and subclinical lesions. Because different genotypes are preferentially associated with benign or malignant lesions, HPV detection and typing is clinically important.
Objective: Our purpose was to assess a polymerase chain reaction (PCR)-based, noninvasive procedure for HPV diagnosis and evaluate the reliability of the acetic acid test for revealing subclinical HPV lesions.
Methods. Mucosal samples were collected by gentle scraping, and PCR-positive samples were typed by hybridization with specific DNA probes.
Results: Seventy-eight men were assessed. The PCR procedure was reliable and easy to perform No significant difference was found in the prevalence of HPV infection between patients whose results were acetowhite-positive and those whose results were acetowhite-negative.
Conclusion: Detection of acetowhite epithelium, although useful for clinical examination, is not sufficiently specific and should not be used as a sole criterion for the diagnosis of HPV infection.

• 出版日期1993-5