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摘要
A micro-electromembrane extraction (mu-EME) technique using electrically induced transfer of charged analytes across free liquid membranes (FLMs) was presented. A disposable extraction unit was proposed and it was made of a short segment of transparent perfluoroalkoxy tubing, which was successively filled with three liquid plugs serving as acceptor solution, FLM and donor solution. These plugs formed a three-phase extraction system, which was precisely defined, that was stable and required mu L to sub-mu L volumes of all respective solutions. Basic instrumental set-up and extraction principles of mu-EME were examined using an anionic and a cationic dye, 4,5-dihydroxy-3-(p-sulfophenylazo)-2,7-naphthalene disulfonic acid trisodium salt (SPADNS) and crystal violet, respectively. Transfers of the charged dyes from donor into acceptor solutions across FLMs consisting of 1-pentanol were visualized by a microscope camera and quantitative measurements were performed by UV vis spectrophotometry. The effects of operational parameters of mu-EME system were comprehensively investigated and experimental measurements were accompanied with theoretical calculations. Extraction recoveries above 60% were achieved for 5 min mu-EME of 1 mM SPADNS at boy with repeatability values below 5%. Selectivity of FLMs was additionally examined by capillary electrophoretic analyses of acceptor solutions and the potential of FLMs for mu-EME pretreatment of samples with artificial complex matrices was demonstrated.
- 出版日期2014-6-13
