Antiviral Activity of Recombinant Porcine Interferon-alpha Against Porcine Transmissible Gastroenteritis Virus in PK-15 Cells

作者：Zhao, Jun; Yi, Xing-xu; Yu, Hai-yang; Wang, Ming-li^*; Lai, Peng-fei; Gan, Lin; Zhao, Yu; Fu, Xiu-le; Chen, Jason^*

来源：Kafkas Universitesi Veteriner Fakultesi Dergisi, 2017, 23(4): 603-611.

DOI：10.9775/kvfd.2017.17462

• Recombinant porcine interferon-alpha (rPoIFN-alpha)
• Porcine transmissible gastroenteritis virus (TGEV)
• Immunofluorescence assay
• Real-time qRT-PCR
• TCID50 assay
• Western blot

A recombinant porcine interferon alpha (rPoIFN-alpha) has been developed and patented previously (Chinese patent number ZL200810020180.4). In the current study, we investigated the inhibitory effects of the rPoIFN-alpha on the propagation of porcine transmissible gastroenteritis virus (TGEV) at different doses in porcine kidney cell line (PK-15). To quantitatively determine the inhibition of viral growth by rPoIFN-alpha, TCID50 assay, plaque formation assay, real-time qRT-PCR, western blot and immunofluorescence assay were adopted to evaluate the changes of viral infectious particles, viral genome copy numbers and viral protein expression levels respectively. The results demonstrated that all the three batches of the rPoIFN-alpha tested inhibited TGEV-induced cytopathic effect in PK-15 cells with very similar potency. rPoIFN-alpha inhibited TGEV proliferation more strongly than human IFN-alpha product. The inhibitory activity of rPoIFN-alpha on TGEV growth in culture was dose dependent, and the activity was gradually reduced with the decreasing of the concentration of rPoIFN-alpha.

• 出版日期2017-8
• 单位安徽医科大学