Anti-Inflammatory Effect of Ascochlorin in LPS-Stimulated RAW 264.7 Macrophage Cells Is Accompanied With the Down-Regulation of iNOS, COX-2 and Proinflammatory Cytokines Through NF-B, ERK1/2, and p38 Signaling Pathway

作者：Lee Sook Hyun; Kwak Choong Hwan; Lee Sung Kyun; Ha Sun Hyung; Park Junyoung; Chung Tae Wook; Ha Ki Tae; Suh Suk Jong; Chang Young Chae; Chang Hyeun Wook; Lee Young Choon; Kang Bong Seok; Magae Junji; Kim Cheorl Ho^*

来源：Journal of Cellular Biochemistry, 2016, 117(4): 978-987.

DOI：10.1002/jcb.25383

• ASCOCHLORIN
• INFLAMMATION
• LPS
• RAW 264
• 7 MACROPHAGE CELLS

A natural compound C23H32O4Cl, ascochlorin (ASC) isolated from an incomplete fungus, Ascochyta viciae has been known to have several biological activities as an antibiotic, antifungal, anti-cancer, anti-hypolipidemic, and anti-hypertension agent. In this study, anti-inflammatory activity has been investigated in lipopolysaccharide (LPS)-induced murine macrophage RAW 264.7 cells, since ASC has not been observed on the inflammatory events. The present study has clearly shown that ASC (1-50M) significantly suppressed the production of nitric oxide (NO) and prostaglandin E-2 (PGE(2)) and decreased the gene expression of inducible NO synthase (iNOS) and cyclooxygenase-2 (COX-2) in a dose-dependent manner. Moreover, ASC inhibited the mRNA expression and the protein secretion of interleukin (IL)-1 and IL-6 but not tumor necrosis factor (TNF)- in LPS-stimulated RAW 264.7 macrophage cells. In addition, ASC suppressed nuclear translocation and DNA binding affinity of nuclear factor-B (NF-B). Furthermore, ASC down-regulated phospho-extracellular signal-regulated kinase 1/2 (p-ERK1/2) and p-p38. These results demonstrate that ASC exhibits anti-inflammatory effects in RAW 264.7 macrophage cells. J. Cell. Biochem. 117: 978-987, 2016.

• 出版日期2016-4