Molecular cloning, expression and characterization of a chitosanase from Microbacterium sp.

作者：Zhang Jiquan; Sun Yuying^*

来源：Biotechnology Letters, 2007, 29(8): 1221-1225.

DOI：10.1007/s10529-007-9373-y

• chitosanase
• chitooligosaccharides
• chitosan
• cloning
• Microbacterium sp.

A gene encoding a chitosanase (mschito) was cloned from Microbacterium, sp. OU01. The ORF consists of 801 bp which encoded a polypeptide of 266 amino acid residues. The deduced amino acid sequence shows 98% identity to that of the chitosanase reported in Pseudomonas sp. A-01. In addition, the fusion protein containing MSCHITO was expressed in E. coli and purified using Ni-NTA affinity chromatography. The purified rMSCHITO protein degraded the chitosan (the degree of deacetylation of 99%) and produced a mixture of chitooligosaccharides. The MSCHITO is thus an endo-chitosanase.

• 出版日期2007-8
• 单位中国科学院海洋研究所; 淮海工学院