A rapid and cheap protocol for preparation of PCR templates in peanut

作者:Wang Chuan Tang; Wang Xiu Zhen; Tang Yue Yi; Zhang Jian Cheng; Yu Shan Lin; Xu Jian Zhi; Bao Zhen Min*
来源:Electronic Journal of Biotechnology, 2009, 12(2): 11.
DOI:10.2225/vol12-issue2-fulltext-11

摘要

This paper describes a simple, low cost and reliable DNA template preparation protocol for polymerase chain reaction (PCR) using immature leaves from peanut seeds or leaves from field-grown plants. The technique may find wide utility in studies involving PCR-based molecular markers, rapid screening for transformants and gene cloning.