Healing of rabbit calvarial bone defects using biphasic calcium phosphate ceramics made of submicron-sized grains with a hierarchical pore structure

作者：Park Jin Woo^*; Kim Eun Suk; Jang Je Hee; Suh Jo Young; Park Kwang Bum; Hanawa Takao

来源：Clinical Oral Implants Research, 2010, 21(3): 268-276.

DOI：10.1111/j.1600-0501.2009.01846.x

• biphasic calcium phosphate
• bone substitute
• histomorphometry
• pore structure
• submicron grain

Objectives
This study investigated the efficacy of new bone graft substitutes - biphasic calcium phosphates (BCP) made of submicron-sized grains with fully interconnected wide-range micron-scale pores in two different macrodesigns: donut shaped with a 300-400 mu m central macropore (n-BCP-1) or rod-shaped (n-BCP-2) - in the healing of rabbit calvarial defects, and compared their bone-healing properties with those of various commercial bone substitutes, which included substitutes with similar BCP composition (MBCP and Osteon), anorganic bovine bone (Bio-Oss), and beta-TCP (Cerasorb).
Material and methods
The surface morphology of the bone substitutes was investigated using scanning electron microscopy (SEM). Defects 8 mm in diameter were created in the calvaria of 30 adult male New Zealand White rabbits and were filled with six types of bone substitutes. The percentage of newly formed bone (NB%) was evaluated histomorphometrically 4 and 8 weeks after implantation.
Results
SEM observation showed submicron-sized grains with fully interconnected micropore structures in the n-BCP-1 and n-BCP-2 groups; these groups also showed considerable new bone formation in inner micropores as well as on the outer surfaces. The n-BCP-1 group exhibited enhanced new bone formation and direct ingrowth of bone tissue with blood vessels into central pores. Histomorphometric analysis showed significantly greater NB% in the n-BCP-1 group when compared with the other groups at 4 and 8 weeks (P < 0.05).
Conclusion
A new BCP ceramics made of submicron-sized grains with a hierarchical pore structure was an effective osteoconductive material for the treatment of osseous defects of rabbit calvaria.
To cite this article:Park J-W, Kim E-S, Jang J-H, Suh J-Y, Park K-B, Hanawa T. Healing of rabbit calvarial bone defects using biphasic calcium phosphate ceramics made of submicron-sized grains with a hierarchical pore structure.Clin. Oral Impl. Res. 21, 2010; 268-276.doi: 10.1111/j.1600-0501.2009.01846.x.

• 出版日期2010-3