Scanning electron microscopy of Streptomyces without use of any chemical fixatives

作者：Kumar Vijay; Bharti Alpana; Gusain Omprakash; Bisht Gajraj Singh^*

来源：Scanning, 2011, 33(6): 446-449.

DOI：10.1002/sca.20261

• fixatives
• scanning
• stubs
• Streptomyces

A new, short, and quick method was developed for preparation of specimen for observing Actinomycetes of genus Streptomyces by scanning electron microscopy. The cultures were directly grown on stubs and coated with a film of gold without using any fixative and dehydrating procedures. Using this simple preparation procedure, surface of intact sporing structures of Streptomyces was observed over a range of magnifications. As the preparation procedure is so simple and rapid, this procedure could be most useful for the routine examination and identification of Streptomyces. SCANNING 33:446449, 2011.

• 出版日期2011-12