Molecular identification of Na plus -H plus exchanger isoforms (NHE2) in the gills of the euryhaline teleost Fundulus heteroclitus

作者：Edwards S L^*; Weakley J C; Diamanduros A W; Claiborne J B

来源：Journal of Fish Biology, 2010, 76(2): 415-426.

DOI：10.1111/j.1095-8649.2009.02534.x

• acid-base regulation
• gills
• Na plus -H plus exchanger
• teleost

In the current study, reverse-transcription polymerase chain reaction (RT-PCR) and rapid amplification of cDNA ends (RACE) PCR were used to clone full-length putative Na+-H+ exchanger isoforms (NHE2a) cDNA from the gills of Fundulus heteroclitus. The 2480 bp cDNA includes a coding region for a protein that shows a 57% amino acid homology to rabbit NHE2. These sequences allowed data mining of available fish genome data, which revealed at least three NHE2 subtypes in some teleost species.

• 出版日期2010-2