Altered dipeptidyl peptidase IV and prolyl endopeptidase activities in chronic tonsillitis, tonsillar hyperplasia and adenoid hyperplasia

作者：Larrinaga Gorka; Perez Itxaro; Sanz Begona; Zarrazquin Idoia; Casis Luis; Antonio Anta Jose; Martinez Agustin; Santaolalla Francisco^*

来源：International Journal of Pediatric Otorhinolaryngology, 2011, 75(3): 347-350.

DOI：10.1016/j.ijporl.2010.11.028

• Dipeptidyl peptidase IV
• Prolyl endopeptidase
• Peptidase
• Chronic tonsillitis
• Tonsillar hyperplasia
• Adenoid hyperplasia
• Tonsillectomy
• Adenoidectomy

Objective: To analyse peptidase activities in the removed tonsils and adenoids from patients with chronic tonsillitis, tonsillar hyperplasia and adenoid hyperplasia.
Methods: we have analyzed 48 tissue samples from patients undergoing tonsillectomy and adenoidectomy for chronic tonsillitis, tonsillar hyperplasia or adenoid hyperplasia. Tonsillectomy and adenoidectomy samples were collected and frozen for later enzyme analysis. The catalytic activity of a pool of peptidases (dipeptidyl peptidase IV, prolyl endopeptidase, aminopeptidase A, aminopeptidase N, aspartyl aminopeptidase, aminopeptidase B. neutral endopeptidase, pyroglutamyl peptidase I, puromycin-sensitive aminopeptidase and cystinyl aminopeptidase) was measured fluorometrically.
Results: The activity of prolyl endopeptidase was higher in tonsillar hyperplasia and adenoid hyperplasia than in chronic tonsillitis. On the contrary, dipeptidyl peptidase IV activity was higher in chronic tonsillitis than in hypertrophic tissues. When data were stratified by age and gender, dipeptidyl peptidase IV was also found to be more active in adult and male chronic tonsillitis tissues. Inversely, dipeptidyl peptidase IV activity was higher in tissues of females with tonsillar hyperplasia.
Conclusions: These data indicate the involvement of dipeptidyl peptidase IV and prolyl endopeptidase in the mechanisms underlying chronic tonsillitis, tonsillar hyperplasia and adenoid hyperplasia.

• 出版日期2011-3