AN M-13-PHAGE LIBRARY DISPLAYING RANDOM 38-AMINO-ACID PEPTIDES AS A SOURCE OF NOVEL SEQUENCES WITH AFFINITY TO SELECTED TARGETS

作者：KAY BK^*; ADEY NB; HE YS; MANFREDI JP; MATARAGNON AH; FOWLKES DM

来源：Gene, 1993, 128(1): 59-65.

DOI：10.1016/0378-1119(93)90153-T

• RECOMBINANT DNA
• PHAGE DISPLAY
• RANDOM PEPTIDE LIBRARY
• EPITOPE MAPPING
• IMMUNOGLOBULINS
• STREPTAVIDIN

We have examined the potential of isolating novel ligands from a library of M 1 3 pIII-fusion phage displaying peptides composed of 38 random amino acids (aa). The library was panned with streptavidin (SA) and a polyclonal goat anti-mouse IgG Fc antibody (Ab) preparation coupled to paramagnetic beads. SA selected two classes of phage from the library. One class exhibited the aa motif, HP(Q/M)THETA (where THETA signifies a non-polar aa), similar to the motif identified by Devlin et al. [Science 249 (1990) 404-406] using a 15-aa random peptide library displayed on phage. The other class of phage had no discernible motif In binding experiments, the non-HP(Q/M)THETA phage had a slightly higher affinity for SA than did the motif phage. Both classes of SA-binding phage failed to bind native and non-glycosylated forms of avidin, even though SA and avidin are structurally similar and both proteins possess extraordinary affinities for biotin. The polyclonal goat anti-mouse IgG Fc Ab preparation selected phage displaying sequences similar to a region of the mouse IgG Fc. Thus, a single immunodominant epitope on the mouse IgG Fc was identified. Furthermore, a second phage displaying peptides with no discernible sequence similarities to mouse IgG Fc was isolated. Thus, an M13 library displaying 38-aa peptides can yield phage with affinity for various targets. Finally, we have observed a biological bias against odd numbers of Cys residues in the displayed peptides.

• 出版日期1993-6-15