Nucleus-encoded mRNAs for Chloroplast Proteins GapA, PetA, and PsbO are Trans-spliced in the Flagellate Euglena gracilis Irrespective of Light and Plastid Function

作者：Mateasikova Kovacova Bianka; Vesteg Matej^*; Drahovska Hana; Zahonova Kristina; Vacula Rostislav; Krajcovic Juraj

来源：Journal of Eukaryotic Microbiology, 2012, 59(6): 651-653.

DOI：10.1111/j.1550-7408.2012.00634.x

• bleaching
• secondary endosymbiosis
• SL-RNA
• trans-splicing
• white mutant

Euglena gracilis is a fresh-water flagellate possessing secondary chloroplasts of green algal origin. In contrast with organisms possessing primary plastids, mRNA levels of nucleus-encoded genes for chloroplast proteins in E. gracilis depend on neither light nor plastid function. However, it remains unknown, if all these mRNAs are trans-spliced and possess spliced leader sequence at the 5%26apos;-end and if trans-splicing depends on light or functional plastids. This study revealed that polyadenylated mRNAs encoding the chloroplast proteins glyceraldehyde-3-phosphate dehydrogenase (GapA), cytochrome f (PetA), and subunit O of photosystem II (PsbO) are trans-spliced irrespective of light or plastid function.

• 出版日期2012-12