Produ o de fator de necrose tumoral-alfa e per車xido de hidrog那nio na infec o pelo Trypanosoma cruzi em camundongos submetidos ao exerc赤cio = Production of tumoral necrosis factor-alpha and hydrogen peroxide during Trypanosoma cruzi infection in mice submitted to exercise

作者：Cristiano Schebeleski Soares; Roberta Cristhiany Occhi; Luana Gomes Luz de Carvalho; Solange Marta Franzoi de Moraes

来源：Acta Scientiarum - Health Sciences, 2010.

• exercicio fisico
• Trypanosoma cruzi
• TNF-alfa
• H2O2
• physical
• exercise
• TNF-alpha

Este trabalho teve como objetivo determinar os n赤veis de fator de necrose tumoral alfa (TNF-alfa) s谷rico e produ o de per車xido de hidrog那nio (H2O2) por macr車fagos peritoneais em camundongos experimentalmente infectados pelo Trypanosoma cruzi e submetidos ao treinamento f赤sico realizado antes da infec o ou ao exerc赤cio f赤sico durante o per赤odo de infec o. Foram utilizados camundongos BALB/c f那meas com 30 dias de idade, inoculados com 1.400 tripomast赤gotas sangu赤neos da cepa Y. O exerc赤cio f赤sico consistia em atividade de intensidade moderada em esteira rolante. As dosagens foram realizadas com material coletado no 13o dia de infec o. Para a dosagem de TNF-alfa, foi utilizada a t谷cnica de ELISA de captura. A produ o de H2O2 foi expressa por colora o produzida ap車s a incuba o de macr車fagos peritoneais com peroxidase e a leitura realizada em aparelho de ELISA. N o foram encontradas diferen as significativas nas concentra es de TNF-alfa e produ o de H2O2 entre os grupos infectados. O treinamento f赤sico realizado antes da infec o e o exerc赤cio f赤sico realizado ap車s a infec o n o foram capazes de alterar significativamente os n赤veis de TNF-alfa e a produ o de H2O2 na infec o pelo T. cruzi. This study aimed to determine the levels of seric tumor necrosis factor-alpha (TNF-alpha) and production of hydrogen peroxide (H2O2) by peritoneal macrophages in mice experimentally infected with T. cruzi and submitted to pre-infection exercise training and to post-infection acute exercise. Female 30-day-old BALB/c mice were inoculated with 1,400 blood trypomastigotes of Y-strain T. cruzi. Exercise programs consisted in moderate-intensity activity and were carried out in a treadmill. The measurements were performed with material collected at the 13th day after infection Serum TNF-alpha was evaluated using capture ELISA. H2O2 production was expressed by coloration produced after incubation of peritoneal macrophages and the measurement was performed using an ELISA reader. There were no statistically significant differences in TNF-alpha levels and H2O2 production between the trained and non-trained infected groups. Thus, the physical training performed before infection and physical exercise performed after the infection were not able to change the levels of TNF-alpha and production of H2O2 in the infection by T. cruzi.