Deciphering the Positional Influence of the Hydroxyl Group in the Cinnamoyl Part of 3-Hydroxy Flavonoids for Structural Modification and Their Interaction with the Protonated and B Form of Calf Thymus DNA Using Spectroscopic and Molecular Modeling Studies

作者：Pradhan Ankur Bikash; Haque Lucy; Bhuiya Sutanwi; Ganguly Aniruddha; Das Suman^*

来源：Journal of Physical Chemistry B, 2015, 119(23): 6916-6929.

DOI：10.1021/acs.jpcb.5b02827

Studies on-the interaction Of naturally occurring flavonoids With different polymorphic forms of nucleic acid are helpful for understanding, the molecular aspects of binding mode and providing direction for the use and design of new efficient therapeutic agents. However, much less information is available Oil the interactions of these compounds with different polymorphic forms of DNA at the molecular level. In this report we investigated the interaction of two widely abundant dietary flavonoids quercetin (Q) and morin (M) with calf thymus (CT) DNA. Spectrophotometric, spectropolarimetric, measurement, and molecular docking simulation methods are used as tools to delineate the binding mode and,probable Ideation of the flavonoids and, their effects on the stability and conformation of DNA. It is observed that in the presence of the protonated form of DNA the,dual fluorescence of Q and M resulting from the excited state intramolecular proton transfer (ESIPT) is modified significantly. Structural analysis, showed Q and M binds weakly to the B forth (groove binding) compared to the protonated form of CT DNA (electrostatic interaction). In both cases) Q binds strongly to both forms of DNA compared to M.

• 出版日期2015-6-11