An improved method for in vitro culture of glochidia in freshwater mussel Cristaria plicata (Mollusca, Bivalvia)

作者：Ma, Xue-Yan; Wen, Hai-Bo; Zou, Jun; Jin, Wu; Hua, Dan; Gu, Ruo-Bo^*; Xu, Pao^*

来源：Hydrobiologia, 2018, 810(1): 133-144.

DOI：10.1007/s10750-016-3012-3

• Non-parasitic metamorphosis
• Metamorphosis rate
• Nutrition factor
• Juvenile mussel culture

We established an improved method for in vitro culture of glochidia in freshwater mussel Cristaria plicata and compared the transformation percentage in L-15 basic medium supplemented with different plasma from host fish or two non-host fish species. The results showed that the metamorphosis rate of glochidia cultured with host fish plasma and non-host fish plasma was all above 96%. High-density lipoprotein and low-density lipoprotein (LDL) levels were significantly lower in host fish plasma than in non-host fish plasma (P < 0.05), suggesting that LDL may be an important nutrient for glochidia metamorphosis. There were no significant differences in the 15-day survival and growth of juvenile mussels obtained from groups with non-host fish plasma under 1100/dish (C5) and a high concentration of antibiotics (D7), as compared with the control (A4) (P > 0.05). The method developed herein not only provides a simplified in vitro culture method for glochidia at a reduced cost, but it also improves the culture efficiency. The method and medium composition may be further improved in studies investigating the long-term growth of juvenile mussels, and it would greatly benefit artificial breeding and germplasm resource preservation for this important species.

• 出版日期2018-3
• 单位中国水产科学研究院; 南京农业大学; 中国水产科学研究院淡水渔业研究中心