Comparative studies on osmosis based encapsulation of sodium diclofenac in porcine and outdated human erythrocyte ghosts

作者：Bukara Katarina^*; Drvenica Ivana; Ilic Vesna; Stancic Ana; Misic Danijela; Vasic Borislav; Gajic Rados; Vucetic Dusan; Kiekens Filip; Bugarski Branko

来源：Journal of Biotechnology, 2016, 240: 14-22.

DOI：10.1016/j.jbiotec.2016.10.017

• Pharmaceutical biotechnology
• Slaughterhouse blood
• Erythrocyte membranes
• Encapsulation
• Gradual hypotonic hemolysis
• Controlled drug delivery

The objective of our study was to develop controlled drug delivery system based on erythrocyte ghosts for amphiphilic compound sodium diclofenac considering the differences between erythrocytes derived from two readily available materials - porcine slaughterhouse and outdated transfusion human blood. Starting erythrocytes, empty erythrocyte ghosts and diclofenac loaded ghosts were compared in terms of the encapsulation efficiency, drug releasing profiles, size distribution, surface charge, conductivity, surface roughness and morphology. The encapsulation of sodium diclofenac was performed by an osmosis based process - gradual hemolysis. During this process sodium diclofenac exerted mild and delayed antihemolytic effect and increased potassium efflux in porcine but not in outdated human erythrocytes. FTIR spectra revealed lack of any membrane lipid disorder and chemical reaction with sodium diclofenac in encapsulated ghosts. Outdated human erythrocyte ghosts with detected nanoscale damages and reduced ability to shrink had encapsulation efficiency of only 8%. On the other hand, porcine erythrocyte ghosts had encapsulation efficiency of 37% and relatively slow drug release rate. More preserved structure and functional properties of porcine erythrocytes related to their superior encapsulation and release performances, define them as more appropriate for the usage in sodium diclofenac encapsulation process.

• 出版日期2016-12-20
• 单位河北医科大学; 中国人民解放军军事医学科学院