Mucin-like Region of Herpes Simplex Virus Type 1 Attachment Protein Glycoprotein C (gC) Modulates the Virus-Glycosaminoglycan Interaction

作者：Altgarde Noomi; Eriksson Charlotta; Peerboom Nadia; Phan Xuan Tuan; Moeller Stephanie; Schnabelrauch Matthias; Svedhem Sofia; Trybala Edward; Bergstrom Tomas; Bally Marta^*

来源：JOURNAL OF BIOLOGICAL CHEMISTRY, 2015, 290(35): 21473-21485.

DOI：10.1074/jbc.M115.637363

• carbohydrate-binding protein
• glycoprotein
• glycosaminoglycan
• glycosylation
• herpesvirus
• surface plasmon resonance (SPR)
• mucin-like region

Background: Herpes simplex virus attachment protein gC comprises a glycosaminoglycan-binding site and a mucin-like region. Results: Removal of the mucin-like region modifies gC interaction with glycosaminoglycans. Conclusion: The mucin-like region balances the gC-glycosaminoglycan interaction during virus binding to and release from target cells. Significance: The finding might be of relevance for similar proteins on other GAG-binding viruses.
Glycoprotein C (gC) mediates the attachment of HSV-1 to susceptible host cells by interacting with glycosaminoglycans (GAGs) on the cell surface. gC contains a mucin-like region located near the GAG-binding site, which may affect the binding activity. Here, we address this issue by studying a HSV-1 mutant lacking the mucin-like domain in gC and the corresponding purified mutant protein (gCmuc) in cell culture and GAG-binding assays, respectively. The mutant virus exhibited two functional alterations as compared with native HSV-1 (i.e. decreased sensitivity to GAG-based inhibitors of virus attachment to cells and reduced release of viral particles from the surface of infected cells). Kinetic and equilibrium binding characteristics of purified gC were assessed using surface plasmon resonance-based sensing together with a surface platform consisting of end-on immobilized GAGs. Both native gC and gCmuc bound via the expected binding region to chondroitin sulfate and sulfated hyaluronan but not to the non-sulfated hyaluronan, confirming binding specificity. In contrast to native gC, gCmuc exhibited a decreased affinity for GAGs and a slower dissociation, indicating that once formed, the gCmuc-GAG complex is more stable. It was also found that a larger number of gCmuc bound to a single GAG chain, compared with native gC. Taken together, our data suggest that the mucin-like region of HSV-1 gC is involved in the modulation of the GAG-binding activity, a feature of importance both for unrestricted virus entry into the cells and release of newly produced viral particles from infected cells.

• 出版日期2015-8-28