Differential gene expression in incompatible interaction between Lilium regale Wilson and Fusarium oxysporum f. sp lilii revealed by combined SSH and microarray analysis

作者：Rao, J.; Liu, D.^*; Zhang, N.; He, H.; Ge, F.; Chen, C.

来源：Molecular Biology, 2014, 48(6): 802-812.

DOI：10.1134/S0026893314060144

• Lilium regale Wilson
• defense response
• Fusarium oxysporum f. sp lilii
• oligonucleotide microarray
• suppression subtractive hybridization

Fusarium wilt, caused by a soilborne pathogen Fusarium oxysporum f. sp. lilii, is the major disease of lily (Lilium L.). In order to isolate the genes differentially expressed in a resistant to F. oxysporum reaction in L. regale Wilson, a cDNA library was constructed with L. regale root during F. oxysporum infection using the suppression subtractive hybridization (SSH), and a total of 585 unique expressed sequence tags (ESTs) were obtained. Furthermore, the gene expression profiles in the incompatible interaction between L. regale and F. oxysporum were revealed by oligonucleotide microarray analysis of 585 unique ESTs in comparison to the compatible interaction between a susceptible Lilium Oriental Hybrid 'Siberia' and F. oxysporum. The result of expression profile analysis indicated that the genes encoding pathogenesis-related proteins (PRs), antioxidative stress enzymes, secondary metabolism enzymes, transcription factors, signal transduction proteins as well as a large number of unknown genes were involved in the early defense response of L. regale to F. oxysporum infection. Moreover, the following quantitative reverse transcription PCR (QRT-PCR) analysis confirmed reliability of the oligonucleotide microarray data. In the present study, isolation of differentially expressed genes in L. regale during response to F. oxysporum helped to uncover the molecular mechanism associated with the resistance of L. regale against F. oxysporum.

• 出版日期2014-11
• 单位昆明理工大学