Anisakis pegreffii: A quantitative fluorescence PCR assay for detection in situ

作者：Fang Wenzhen; Liu Fan; Zhang Shaolei; Lin Junhua; Xu Shisan; Luo Damin^*

来源：Experimental Parasitology, 2011, 127(2): 587-592.

DOI：10.1016/j.exppara.2010.11.008

• Anisakis pegreffii
• Food-borne parasitic zoonosis
• In situ
• Nematode
• Second internal transcribed spacer (ITS-2)
• TaqMan real-time PCR

To facilitate improved diagnosis and detection of the third stage larva (L3) of Anisakis pegreffii from the Minnan-Taiwan bank fishing ground in Taiwan Strait, a real-time PCR method for the detection in situ and differentiation was developed to amplify a region of the second internal transcribed spacer (ITS-2) of this parasite. The real-time PCR assay was capable of detecting 1/3 of a single 13 in 30 mg of marine fish tissue, and also exhibited a high level of specificity for A. pegreffii, no fluorescence signals were observed in other five major larval anisakid species found in commercial marine fishes caught in this fishing ground.

• 出版日期2011-2
• 单位厦门大学