A novel heme oxygenase-1 splice variant, 14kDa HO-1, promotes cell proliferation and increases relative telomere length

作者：Bian, Chunxiang; Zhong, Maojiao; Nisar, Muhammad Farrukh; Wu, Yan; Ouyang, Mingyue; Bartsch, Joerg W.; Zhong, Julia Li^*

来源：Biochemical and Biophysical Research Communications, 2018, 500(2): 429-434.

DOI：10.1016/j.bbrc.2018.04.096

• HO-1
• Alternative splicing
• 14 kDa HO-1
• Telomere length
• Cell proliferation

Alternative splicing is a routine phenomenon which greatly increases the diversity of proteins in eukaryotic cells. In humans, most multi-exonic genes are alternatively spliced and their splice variants confer distinct functions. Heme oxygenase-1 (HO-1, 32 kDa) is an inducible stress responsive protein, which possesses multiple functions in many cellular processes. In the current study, we identified a novel alternative splice isoform of 14 kDa HO-1 generated through exclusion of exon 3, and it is highly expressed in immortalized cells. In contrast to nuclear accumulation of the full-length 32 kDa HO-1, the novel 14 kDa HO-1 isoform is retained in the cytoplasm under ultraviolet (UV) irradiation. Interestingly, the 14 kDa HO-1 is shown to promote cell proliferation and an increase in relative telomere lengths in vivo and in vitro. Thus, we are pioneer to report and confirm the presence of a novel splice form of HO-1 and its distinct role in modulating telomere length and tumor growth.

• 出版日期2018-6-2
• 单位重庆大学