Anti-inflammatory effects of three kinds of traditional Mongolian medicine monomer and its combination on LPS-stimulated RAW264.7 macrophages

作者：Huang, L. -H.; Pan, X. -P.^*; Gong, K. -R.; Shao, G.^*

来源：European Review for Medical and Pharmacological Sciences, 2016, 20(5): 950-958.

• Anti-inflammation
• Traditional Mongolian medicine
• Cytokines
• NF-kappa B
• TLR 4
• LPS

OBJECTIVE: Traditional Mongolian Medicine (TMM) exhibits useful biological activities including antifungal, antibacterial, and anti-inflammatory actions. The mechanisms of TMM in anti-inflammation were still unclear. The aim of this study was to investigate the effects of the three main monomers (geniposide, gallate, berberine hydrochloride and a mixture of them) of a traditional Mongolian medicine on cell survival and the proinflammatory cytokines signaling pathways which are activated by bacterial lipopolysaccharides (LPS).
MATERIALS AND METHODS: Mouse macrophage-like cell line RAW264.7 was used as a model of inflammation to investigate the anti-inflammatory effects of three TMM momomers and their combination. RT-PCR and Western blot was used to quantify the change of mRNA and protein levels of cytokines, Toll-like receptor-4 (TLR4) and Nuclear Factor-kappa B (NF-kappa B) and its inhibitor I kappa B. The non-radioactive electrophoresis mobility shift assay (EMSA) was used to evaluate the binding activity of NF-kappa B.
RESULTS: The monomers and their combination exhibited a potent anti-inflammatory effect for suppressing the LPS-evoked secretion of proinflammatory cytokines IL-1 beta, IL-6 and TNF alpha. Furthermore, the monomers and their combination attenuated activation of NF-kappa B and expression of TLR4 at both mRNA and protein levels, the upstream player of the LPS-TLR4-cytokines/NF-kappa B signaling pathway.
CONCLUSIONS: The Mongolia herbal compound exerts a potent anti-inflammatory effect and could potentially be developed as a useful agent for the chemo-prevention of inflammatory diseases.

• 出版日期2016-3
• 单位内蒙古科技大学包头医学院