Effect of increased strut porosity of calcium phosphate bone graft substitute biomaterials on osteoinduction

作者：Coathup Melanie J^*; Hing Karin A; Samizadeh Sorousheh; Chan Oliver; Fang Yvette S; Campion Charlie; Buckland Thomas; Blunn Gordon W

来源：Journal of Biomedical Materials Research Part A, 2012, 100A(6): 1550-1555.

DOI：10.1002/jbm.a.34094

• bone regeneration
• silicon
• calcium phosphate
• porosity
• animal model

The effect of increasing strut porosity on the osteoinductivity of porous calcium phosphate (CaP) and silicate-substituted calcium phosphate (SiCaP) bone substitute materials was investigated in an ovine ectopic model. One to two millimeter-sized granules or block implants with strut porosities of 10, 20, or 30% were inserted into the left and right paraspinalis muscle. At 12 weeks, histological sections were prepared through the center of each implant and bone contact, bone area and implant area quantified. Backscattered scanning electron microscopy (bSEM) was used to visualize bone within small pores in the struts of the scaffolds. Increased bone formation was measured in the SiCaP with 30% strut porosity (5.482% +/- 1.546%) when compared with the nonsilicate CaP with the same morphology (1.160% +/- 0.502%, p = 0.02), indicating that silicate substitution may increase osteoinduction. Greater bone formation was seen in scaffolds with increased strut porosity. No bone growth was found in any of the SiCaP scaffold with 10% porosity. There was no significant difference between block and granule specimens. Scanning electron microscopy and EDX in combination with histology demonstrated bone formation within pores %26lt;5 mu m in size. The use of silicate-substituted CaP material with increased strut porosity may further augment repair and regeneration in bony sites.

• 出版日期2012-6