Age-related changes in cochlear endolymphatic potassium and potential in CD-1 and CBA/CaJ mice

作者：Wu T; Marcus DC^*

来源：Journal of the Association for Research in Otolaryngology, 2003, 4(3): 353-362.

DOI：10.1007/s10162-002-3026-6

• stria vascularis
• endocochlear potential
• cochlea
• potassium concentration
• histology
• age-related hearing loss

The CD-1 mouse strain is known to have early onset of hearing loss that is progressive with aging. We sought to determine whether a disturbance of K homeostasis and pathological changes in the cochlear lateral wall were involved in the age-related hearing loss (AHL) of CD-1 as compared to the CBA/CaJ strain which has minimal AHL. In the present study, the endocochlear potential (EP) and endolymphatic K+ concentration ([K+](e)) were measured in both strains of mice with double-barrel microelectrodes at "young" (1-2 mo) and "old" (5-9 mo) ages. CBA/CaJ mice displayed no changes with aging in EP and [K+](e) of the basal turn. In the apical turn, there was a small positive shift of the EP (10 mV) with aging under both normoxic and acute anoxic conditions (-EP), without any change of [K+](e). Further, there were no obvious pathological changes in the lateral wall of CBA/CaJ mice. By contrast, old CD-I mice displayed a significantly reduced [K+](e) by 30% in both basal and apical turns with no significant changes in normoxic EP. The -EP in the apical turn was significantly reduced in magnitude by 6 mV. A severe loss of cells with aging was observed in the region of type IV fibrocytes of the apical and basal turns and of type II fibrocytes in the basal turn. A complete degeneration of organ of Corti was also observed at the basal turn of old CD-I mice, as well as a basalward decline of spiral ganglion neuron density. The pathological changes in spiral ligament of CD-I mice were similar to those of an inbred mouse strain C57BL/6J that expresses an AHL gene (ahl) and might be a primary etiology of AHL of CD-1 mice. These findings have ramifications for our understanding of AHL and for interpretation of genetic mutations in a CD-1 background.

• 出版日期2003-9