A cancer cell-specific two-photon fluorescent probe for imaging hydrogen sulfide in living cells

作者：Song, Xuezhen; Dong, Baoli; Kong, Xiuqi; Wang, Chao; Zhang, Nan; Lin, Weiying^*

来源：RSC Advances, 2017, 7(26): 15817-15822.

DOI：10.1039/c7ra01479a

• H2S
• SULFHYDRATION
• INFLAMMATION
• MOUSE
• WATER

Hydrogen sulfide (H2S) could induce the proliferation of cancer cells in a concentration-dependent manner, and has close relation with the tumor growth. Monitoring the H2S level in real-time is of great important for understanding its roles in the cancer cell proliferation and the diagnosis of the tumor. Herein, a novel cancer cell-specific two-photon fluorescent probe BN-H2S for detecting H2S in cancer cells was designed and synthesized. Biotin was selected as the cancer cell-specific group and the azide group was employed as the response site for H2S. When BN-H2S responded to H2S, a turn-on fluorescence at 544 nm was observed clearly. BN-H2S exhibited high selectivity for H2S over other relative species. Under the guidance of the biotin group, BN-H2S can be successfully used for the two-photon imaging of H2S in cancer cells, while BN-H2S showed relatively weak response for H2S in normal cells. We expect that this design concept can be further developed for selectively detecting other biomolecules in living cancer cells.

• 出版日期2017
• 单位济南大学