NMR study of Met-1 human Angiogenin: H-1, C-13, N-15 backbone and side-chain resonance assignment

Tsika Aikaterini C; Chatzileontiadou Demetra S M; Leonidas Demetres D<sup>*</sup>; Spyroulias Georgios A<sup>*</sup>

doi:10.1007/s12104-016-9704-9

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NMR study of Met-1 human Angiogenin: H-1, C-13, N-15 backbone and side-chain resonance assignment

作者：Tsika Aikaterini C; Chatzileontiadou Demetra S M; Leonidas Demetres D^*; Spyroulias Georgios A^*

来源：Biomolecular NMR Assignments, 2016, 10(2): 379-383.

DOI：10.1007/s12104-016-9704-9

摘要

Here, we report the high yield expression and preliminary structural analysis via solution hetero-nuclear NMR spectroscopy of the recombinant Met-1 human Angiogenin. The analysis reveals a well folded as well as, a monomeric polypeptide. She sequence-specific assignment of its H-1, N-15 and C-13 resonances at high percentage was obtained. Also, using TALOS+ its secondary structure elements were determined.