A functional protein retention and release multilayer with high stability

作者：Nie, Kun; An, Qi^*; Zhang, Yihe^*

来源：Nanoscale, 2016, 8(16): 8791-8797.

DOI：10.1039/c6nr01671e

• BUILDING-BLOCKS
• CROSS-LINKING
• THIN-FILMS
• CATALASE
• IMMOBILIZATION
• NANOPARTICLES
• CONSTRUCTION
• TRANSITION
• ADSORPTION
• LOCOMOTION

Effective and robust interfacial protein retention lies at the heart of the fabrication of protein-based functional interfaces, which is potentially applicable in catalysis, medical therapy, antifouling, and smart devices, but remains challenging due to the sensitive nature of proteins. This study reports a general protein retention strategy to spatial-temporally confine various types of proteins at interfacial regions. The proteins were preserved in mesoporous silica nanoparticles embedded in covalently woven multilayers. It is worth noting that the protein retention strategy effectively preserves the catalytic capabilities of the proteins, and the multilayer structure is robust enough to withstand the bubbling catalytic reactions and could be repeatedly used due to conservation of proteins. The spatiotemporal retention of proteins could be adjusted by varying the number of capping layers. Furthermore, we demonstrate that the protein-loaded interfacial layers could not only be used to construct catalytic-active interfaces, but also be integrated as the power-generating unit to propel a macroscopic floating device.

• 出版日期2016
• 单位中国地质大学（北京）