Automatic classification of acute and chronic myeloid leukemic cells with wide-angle label-free static cytometry

作者：Xie, Linyan; Liu, Qiao; Shao, Changshun; Su, Xuantao^*

来源：Optics Express, 2017, 25(23): 29365-29373.

DOI：10.1364/OE.25.029365

• LIGHT-SCATTERING PATTERNS
• FLOW-CYTOMETRY
• CROSS-VALIDATION
• SINGLE CELLS
• ORGANIZATION
• ABERRATIONS
• MODEL

A wide-angle 2D light scattering static cytometer is developed for the automatic classification of label-free acute and chronic myeloid leukemic cells. This wide-angle cytometer collects 2D light scattering signals in the angular range from 26 to 154 degrees, which is validated by good agreement between experimental and simulated 2D light scattering patterns from standard microspheres. By combining gray level differential statistics (GLDS) algorithm with support vector machine (SVM) for the 2D light scattering pattern analysis, HL-60 cells (acute myeloid leukemic cells) and K562 cells (chronic myeloid leukemic cells) can be automatically identified with a sensitivity of 92% and a specificity of 95%. Our wide-angle 2D light scattering label-free static cytometry may serve as a new method for the diagnosis and classification of leukemia subtypes.

• 出版日期2017-11-13
• 单位山东大学