Detection of lipid-lysine amide-type adduct as a marker of PUFA oxidation and its applications

作者：Kato Yoji^*; Osawa Toshihiko

来源：Archives of Biochemistry and Biophysics, 2010, 501(2): 182-187.

DOI：10.1016/j.abb.2010.06.010

• Lipid peroxidation
• Amide-type adduct
• N epsilon-(hexanoyl)lysine
• N epsilon-(propanoyl)lysine
• Functional food
• Oxidative stress marker

Research into lipid peroxidation-induced protein modification has been ongoing for many years Recent studies on lipo-oxidation shows the occurrence of another type of protein modification, amide-type adduct formation by lipid hydroperoxide, as well as classical aldehyde-derived protein modifications The amide-type modifications can be either classified as alkylamide and carboxyalkylamide according to the formed structures As an alkylarmde-type adduct, N-epsilon(hexanoyl)lysine can be formed by the reaction of peroxidized n - 6 fatty acid with lysine N epsilon-(propanoyl)lysine is considered to be generated from oxidation of n - 3 fatty acid with lysine The generation pattern of both might be useful for classification of which fatty acids are more involved in oxidation in vivo Since the alkylamide type-adducts are relatively stable and detectable from biological specimens like urine, these

• 出版日期2010-9-15