Ultrasensitive electrochemiluminescence method for determination of DNA using Ru(bpy)(3)(2+)-coated magnetic submicrobeads wrapped with carbon nanotubes

作者：Shen, Liping; Li, Jun; Li, Lu; Zou, Guizheng^*; Zhang, Xiaoli; Jin, Wenrui

来源：Electrochemistry Communications, 2011, 13(12): 1499-1501.

DOI：10.1016/j.elecom.2011.10.002

• Electrochemiluminescence assay
• DNA detection
• Microbead
• Carbon nanotube

We developed an ultrasensitive electrochemiluminescence (ECL) method for DNA determination using magnetic submicrobeads (SMBs) as the carrier of Ru(bpy)(3)(2+) (bpy = 2,2'-bipyridy) and carbon nanotubes (CNTs) as accessorial electrode material. The SMBs with Ru(bpy)(3)(2+) were wrapped with CNTs and then immobilized on an Au electrode. In the presence of tri-n-propylamine, ECL of the Ru(bpy)(3)(2+) on the SMBs was detected. Since one target DNA (t-DNA) molecule corresponded to one SMB with a large number of Ru(bpy)(3)(2+), the ECL signal was amplified. In addition, the Ru(bpy)(3)(2+)-loaded SMBs were wrapped with CNTs that contacted the electrode. The ECL of Ru(bpy)(3)(2+) was greatly increased. Using this method, t-DNA of 3 x 10(-16) mol/L could be detected. The method could be used to quantify mRNA in cells.

• 出版日期2011-12
• 单位山东师范大学; 山东大学