A novel vector for the expression of SCR domains in insect cells

作者：Alam MN; Haque A; Sreedhar M; Pangburn MK^*

来源：Journal of Immunological Methods, 2004, 293(1-2): 107-113.

DOI：10.1016/j.jim.2004.07.005

• vector
• insect cells
• expression
• SCR domains
• CCP domains

Exploitation of recombinant technology to study proteins containing strings of short consensus repeat (SCR) domains largely depends on expression vectors. In this paper, we describe a vector for cloning and constitutive expression of single or multiple SCR domains. The recombinant vector has unique additive features over commercially available vectors that make it a universal cloning vector for SCR domains as well as a vector suitable for expressing any protein fragment beginning and ending with cysteine residues. As a demonstration of its usefulness, the constitutive extracellular expression of five SCR-containing proteins derived from complement factor H is presented.

• 出版日期2004-10