Universal modular system for in vitro screening of potential inhibitors of HIV-1 replication

作者：Prokofjeva M M^*; Orlova N N; Gornostaeva A S; Shulgin A A; Nikitenko N A; Senchenko V N; Lebedev T D; Spirin P V; Riecken K; Fehse B; Stocking C; Prassolov V S

来源：Molecular Biology, 2014, 48(2): 297-300.

DOI：10.1134/S0026893314020150

• HIV
• pseudo-HIV-1 particles
• reverse transcriptase
• nucleoside inhibitors
• non-nucleoside inhibitors
• integrase inhibitors
• sulfated polysaccharides

A system based on recombinant lentiviral vectors has been designed for safe screening of potential anti-HIV drugs. The system can be used to evaluate the sensitivity of HIV-1 reverse transcriptase and integrase (wild-type as well as mutant forms of these enzymes detected in drug-resistant virus isolates) to different drugs and substances, as well as to screen inhibitors of other stages of the HIV-1 life cycle.

• 出版日期2014-3