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摘要
A liquid chromatographic/tandem mass spectrometric method using pneumatically assisted electrospray ionization (LC-ESI-MS/MS) was developed for determination of N-acetylneuramic acid and N-glycolylneuramic acid in infant formula Reconstituted samples were hydrolysed in dilute sulfuric acid and deproteinized with acetonitrile The extract was analysed directly without further clean-up by hydrophilic interaction chromatography The substances were detected in negative ion mode and matrix matched standards were used for calibration The relative intra-laboratory reproducibility standard deviation was better than 6% for both substances An R(2) of 0 985 was obtained by comparison with a clas
- 出版日期2010-11
