科研之友
微信
新浪微博
Facebook
分享链接
摘要
In pH 5.5 2-(N-morpholine)-ethyl sulfonic acid buffer solution and in the presence of NaCl, the substrate single stranded DNA hybridized with the DNA enzyme to form double stranded DNA (dsDNA) at 80 degrees C. UO22+ can cleaved the substrate strand of the dsDNA to produce short single stranded DNA that adsorbed on the surface of the gold nanoparticle to prevent its aggregation, and the uncombined gold nanoparticles aggregate to big particles that exhibited a resonance scattering (RS) peak at 610 nm. When the UO22+ concentration increased, the short single stranded DNA increased, the combined gold nanoparticles increased, and the aggregated gold nanoparticles decreased, the RS intensity at 610 nm decreased. Under the selected conditions, the decreased RS intensity (Delta I-610 (nm)) is linear to UO22+ concentration in the range of 0.67 similar to 60.3 nmol/L, with a regression equation of Delta I-610 (nm)=11.9C+6.1, a correlation coefficient of 0.9972, and a detection limit of 0.09 nmol/L UO22+. This method has been applied to determination of UO22+ in wastewater, with satisfactory results.
- 出版日期2011-9-28
- 单位广西师范大学
