AN EXPANDED NUCLEAR PHYLOGENOMIC PCR TOOLKIT FOR SAPINDALES

作者：Collins Elizabeth S^*; Gostel Morgan R; Weeks Andrea

来源：Applications in Plant Sciences, 2016, 4(12): 1600078.

DOI：10.3732/apps.1600078

• Anacardiaceae
• Burseraceae
• low-copy nuclear genes
• microfluidic PCR
• Rutaceae

Premise of the study: We tested PCR amplification of 91 low-copy nuclear gene loci in taxa from Sapindales using primers developed for Bursera simaruba (Burseraceae).
Methods and Results: Cross-amplification of these markers among 10 taxa tested was related to their phylogenetic distance from B. simaruba. On average, each Sapindalean taxon yielded product for 53 gene regions (range: 16-90). Arabidopsis thaliana (Brassicales), by contrast, yielded product for two. Single representatives of Anacardiaceae and Rutacaeae yielded 34 and 26 products, respectively. Twenty-six primer pairs worked for all Burseraceae species tested if highly divergent Aucoumea klaineana is excluded, and eight of these amplified product in every Sapindalean taxon.
Conclusions: Our study demonstrates that customized primers for Bursera can amplify product in a range of Sapindalean taxa. This collection of primer pairs, therefore, is a valuable addition to the toolkit for nuclear phylogenomic analyses of Sapindales and warrants further investigation.

• 出版日期2016-12