Analysis of chitinase gene specific transcript accumulation in tea [Camellia sinensis (L.) O. Kuntze] during induced systemic resistance by methyl jasmonate

作者：Roy S C^*; Chakraborty B N

来源：Indian Journal of Biotechnology, 2012, 11(2): 142-147.

• Camellia sinensis
• chitinase gene transcript
• ISR
• RT-PCR

Chitinase gene (Pr-3) specific transcript accumulation was increased by 5.57% in mature leaf compared to the other in vitro grown tissues in tea (Camellia sinensis) genotype (T383) during induced systemic resistance by methyl jasmonate. Chitinase (Pr-protein) gene specific two primers were used in RT-PCR reaction to measure the differential transcript accumulation in different tissue systems (young leaf, mature leaf, callus and shoot regenerated from somatic embryo). The time-course expression patterns resulted in differential expression of two transcripts (254 & 366 bp) in response to induction. The 366 bp transcript was sequenced bidirectionally and deposited into the GenBank of NCBI (acc.no. EU373553).

• 出版日期2012-4
• 单位宁波大学