ATR-IR study of skin components: Lipids, proteins and water. Part II: Near infrared radiation effect

作者：Olsztynska Janus S^*; Kielbowicz Z; Czarnecki M A

来源：Spectrochimica Acta Part A: Molecular and Biomolecular Spectroscopy , 2018, 202: 93-101.

DOI：10.1016/j.saa.2018.05.036

• ATR-IR
• Vibrational spectroscopy
• Skin
• Near infrared
• Phase transition
• Denaturation
• Biomedical engineering

Near infrared (NIR) radiation has been widely used in medicine and biomedical engineering. In spite of numerous studies the molecular mechanism of NIR radiation on biological systems has not been established as yet. The objective of this work was examination of the effect of NIR irradiation on the skin components. Modifications of lipid organization after NIR exposure vs. temperature (from 20 to 90 degrees C) have been investigated using Attenuated Total Reflectance Infrared (ATR-IR) spectroscopy. This work is a continuation of our previous studies on the temperature effect on skin components [1]. After NIR exposure a temperature shift of the phase transition from the orthorhombic to hexagonal packing (approximate to 140 degrees C) has been observed. In contrast, the second phase transition temperature (approximate to 70 degrees C) is almost invariable. The phase transitions in lipids were correlated with modifications of the structure of water and proteins. To our knowledge, for the first time the temperatures of the phase transitions after NIR exposure were investigated.

• 出版日期2018-9-5