A polysaccharide from Armillaria mellea exhibits strong in vitro anticancer activity via apoptosis-involved mechanisms

作者：Wu, Jun; Zhou, Jinxu; Lang, Yaoguo; Yao, Lei; Xu, Hai; Shi, Hubo; Xu, Shidong^*

来源：International Journal of Biological Macromolecules, 2012, 51(4): 663-667.

DOI：10.1016/j.ijbiomac.2012.06.040

• Armillaria mellea
• Polysaccharide
• Apoptosis
• Caspase
• Mitochondrial membrane potential

Armillaria mellea is a famous traditional Chinese medicinal and edible fungus. In this study, we purified a water-soluble polysaccharide (AMP) from the fruiting bodies of this fungus. AMP contained 94.8% carbohydrate, 2.3% uronic acid and 0.5% protein. Its molecular weight was determined as 4.6 x 10(5) Da, as determined by high-performance gel-permeation chromatography (HPGPC). Gas chromatography (GC) analysis indicated that AMP was mainly composed of o-glucose. In vitro assay. AMP exhibited a potent tumor growth inhibitory effect on A549 cells, and induced cell cycle disruption in the GO/G1 phase, accompanied by an increment of apoptotic cells. Furthermore, AMP induced the disruption of mitochondrial membrane potential, thus leading to cytochrome c release from mitochondria and activation of caspase-3 and -9. Taken together, our results demonstrate that AMP possesses strong antitumor activities through the mitochondria dependent pathway and activation of caspase cascade through cytochrome c release.

• 出版日期2012-11
• 单位哈尔滨工业大学; 哈尔滨医科大学