Addressable multiregional and multifocal multiphoton microscopy based on a spatial light modulator

作者：Shao, Yonghong; Qin, Wan; Liu, Honghai; Qu, Junle; Peng, Xiang; Niu, Hanben; Gao, Bruce Z^*

来源：Journal of Biomedical Optics, 2012, 17(3): 030505.

DOI：10.1117/1.JBO.17.3.030505

• multiphoton microscopy
• multifocus
• spatial light modulator
• addressable scanning
• dynamic imaging

Through a combination of a deflective phase-only diffractive spatial light modulator (SLM) and galvo scanners, an addressable multiregional and multifocal multiphoton microscope (AM-MMM) is developed. The SLM shapes an incoming mode-locked, near-infrared Ti:sapphire laser beam into multiple beamlet arrays with addressable shapes and sizes that match the regions of interest on the sample. Compared with conventional multifocal multiphoton microscope (MMM), AM-MMM achieves the effective use of the laser power with an increase of imaging rate and a decrease of photodamage without sacrifice of resolution.

• 出版日期2012-3
• 单位深圳大学