Dual role of B cells with accelerated onset but reduced disease activity in P0(106-125)-induced experimental autoimmune neuritis of IgH(0/0) mice

作者：Brunn Anna^*; Utermoehlen Olaf; Sanchez Ruiz Monica; Montesinos Rongen Manuel; Blau Tobias; Schlueter Dirk; Deckert Martina

来源：Acta Neuropathologica, 2010, 120(5): 667-681.

DOI：10.1007/s00401-010-0724-8

• EAN
• Mouse
• B cells
• CD4 and CD8 T cells
• IL-10

The role of B cells in autoimmune-mediated diseases of the peripheral nervous system was studied in experimental autoimmune neuritis (EAN) in B cell deficient IgH(0/0) C57BL/6J mice having been immunized with P0(106-125) peptide. Compared to coisogenic IgH(+/+) mice, onset of EAN was accelerated [100% disease incidence at day 9 post immunization (p.i.) vs. day 15 p.i.]. At day 9 p.i., numbers of P0(106-125)-specific interferon (IFN)-gamma-producing CD4(+) T cells were increased, while IL-10 mRNA and production were decreased in IgH(0/0) mice. Beyond day 9 p.i., declining disease activity and a significant reduction of maximal disease activity were correlated with significantly reduced numbers of IFN-gamma-producing CD4(+) T cells in IgH(0/0) mice as compared with IgH(+/+) mice. Correspondingly, neuropathology demonstrated only mild axonal damage, while demyelination and dying back axonopathy with spinal cord motor neuron apoptosis were absent. Thus, depending on the stage of EAN, B cells play a dual, i.e. suppressive and enhancing, role during induction and at height of EAN, respectively. The combined interaction of B cells as well as CD4(+) and CD8(+) T cells is required for the development of EAN.

• 出版日期2010-11
• 单位河北医科大学