Association of LIPC-250G > A polymorphism and several environmental factors with serum lipid levels in the Guangxi Bai Ku Yao and Han populations

作者：Li Meng; Yin Ruixing^*; Li Yiyang; Long Xingjiang; Li Kela; Liu Wanying; Zhang Lin; Lin Weixiong; Yang Dezhai; Pan Shangling

来源：Lipids in Health and Disease, 2010, 9: 28.

DOI：10.1186/1476-511X-9-28

• HEPATIC LIPASE GENE
• CORONARY-ARTERY-DISEASE
• DENSITY-LIPOPROTEIN CHOLESTEROL
• ISCHEMIC-HEART-DISEASE
• AFRICAN-AMERICAN MEN
• PROMOTER POLYMORPHISMS
• HDL3 SUBFRACTIONS
• CIGARETTE-SMOKING
• BLOOD-LIPIDS
• RISK-FACTORS

Background: The association between -250G>A polymorphism in the promoter region of the hepatic lipase gene (LIPC) and plasma high-density lipoprotein cholesterol (HDL-C) concentration is contradictory in diverse ethnics. Bai Ku Yao is an isolated subgroup of the Yao minority in China. This study was designed to detect the association of LIPC -250G>A (rs2070895) polymorphism and several environmental factors with serum lipid levels in the Guangxi Bai Ku Yao and Han populations.
Methods: A total of 778 subjects of Bai Ku Yao and 648 participants of Han Chinese aged 15-80 were randomly selected from our previous stratified randomized cluster samples. Genotyping of the LIPC -250G>A was performed by polymerse chain reaction and restriction fragment length polymorphism combined with gel electrophoresis, and then confirmed by direct sequencing.
Results: The levels of serum total cholesterol (TC), HDL-C, low-density lipoprotein cholesterol (LDL-C) and apolipoprotein (Apo) AI were lower in Bai Ku Yao than in Han (P < 0.01 for all). The frequencies of GG, GA and AA genotypes were 50.0%, 43.3% and 6.7% in Bai Ku Yao, and 35.7%, 50.6% and 13.7% in Han (P < 0.01); respectively. The frequencies of G and A alleles were 71.7% and 28.3% in Bai Ku Yao, and 61.0% and 39.0% in Han (P < 0.01). The levels of HDL-C and the ratio of ApoAI to ApoB in Bai Ku Yao were lower in GG genotype than in GA or AA genotype (P < 0.05-0.01). The levels of TC, HDL-C, LDL-C and ApoB in Han were lower in GG genotype than in GA or AA genotype (P < 0.05-0.01). The levels of HDL-C and the ratio of ApoAI to ApoB in Bai Ku Yao, and the levels of HDL-C, LDL-C and ApoB in Han were correlated with genotype and/or allele (P < 0.05 for all). Serum lipid parameters were also correlated with age, sex, alcohol consumption, cigarette smoking, blood pressure, body weight, and body mass index in both ethnic groups.
Conclusions: The differences in the serum lipid profiles between the two ethnic groups might partly result from different genotypic frequency of LIPC - 250G>A or different LIPC-enviromental interactions.

• 出版日期2010-3-11
• 单位广西医科大学