Different catalytic behavior of alpha-amylase in response to the nitrogen substance used in the production phase

作者：Hashemi M^*; Shojaosadati S A; Razavi S H; Mousavi S M

来源：Journal of Industrial and Engineering Chemistry, 2015, 21: 772-778.

DOI：10.1016/j.jiec.2014.04.011

• Nitrogen substrate
• alpha-Amylase catalytic performance
• Central composite design

The impact of nitrogen substrate on the alpha-amylase performance at two assay conditions (pH = 4.5, 65 degrees C; pH = 6.8, 55 degrees C) was investigated. The highest alpha-amylase activity difference (similar to 38%) was related to the medium consist of dextrin, meat and yeast extracts at (-1) levels, while the lowest variation (similar to 7%) was recorded in experiment contained the minimum amount of yeast extract followed by one with the lowest concentration of meat extract (similar to 9%). The only medium resulted in lower alpha-amylase activity (27%) at pH = 4.5 was the treatment with meat and yeast extracts at (-1) levels. The effect of C/N ratio on the responses was also investigated.

• 出版日期2015-1-25