Analysis of Trace Microcystins in Vegetables Using Solid-Phase Extraction Followed by High Performance Liquid Chromatography Triple-Quadrupole Mass Spectrometry

作者：Li, Yan-Wen; Zhan, Xiao-Jing; Xiang, Lei; Deng, Zhe-Shen; Huang, Bin-Hui; Wen, Hong-Fei; Sun, Teng-Fei; Cai, Quan-Ying; Li, Hui; Mo, Ce-Hui^*

来源：Journal of Agricultural and Food Chemistry, 2014, 62(49): 11831-11839.

DOI：10.1021/jf5033075

• microcystins
• vegetable
• HPLC-MS/MS
• simultaneous analysis
• matrix effect

A selective and sensitive method for the simultaneous detection of three common and hazardous microcystins (microcystins-LR, -RR, and -YR) in various vegetables was established using solid-phase extraction followed by high performance liquid chromatography coupled with mass spectrometry. The methanol-water proportion ratio of the extraction solvent and its acidity, as well as the efficiencies of solid-phase extraction, were evaluated to optimize a pretreatment procedure for extracting the microcystins from 10 vegetable matrices. The limits of detection and quantitation were below 7.5 mu g/kg (dw) and 25 mu g/kg (dw), respectively, in different vegetable matrices. The recoveries of the microcystins in the 10 vegetable matrices ranged from 61.3 to 117.3%, with RSDs of 0.2-18.3%. The established method was used to analyze 28 field vegetable samples collected from the sides of Lake Dianchi, and microcystin-RR was found in almost all samples at concentrations of 36.4-2352.2 mu g/kg (dw).

• 出版日期2014-12-10
• 单位暨南大学