A microfluidic biochip platform for electrical quantification of proteins

作者：Valera Enrique; Berger Jacob; Hassan Umer; Ghonge Tanmay; Liu Julia; Rappleye Michael; Winter Jackson; Abboud Daniel; Haidry Zeeshan; Healey Ryan; Hung Na Teng; Leung Nathaniel; Mansury Naif; Hasnain Alexander; Lannon Christine; Price Zachary; White Karen; Bashir Rashid^*

来源：Lab on a Chip, 2018, 18(10): 1461-1470.

DOI：10.1039/c8lc00033f

Sepsis, an adverse auto-immune response to an infection often causing life-threatening complications, results in the highest mortality and treatment cost of any illness in US hospitals. Several immune biomarker levels, including Interleukin 6 (IL-6), have shown a high correlation to the onset and progression of sepsis. Currently, no technology diagnoses and stratifies sepsis progression using biomarker levels. This paper reports a microfluidic biochip platform to detect proteins in undiluted human plasma samples. The device uses a differential enumeration platform that integrates Coulter counting principles, antigen specific capture chambers, and micro size bead based immunodetection to quantify cytokines. This microfluidic biochip was validated as a potential point of care technology by quantifying IL-6 from plasma samples (n = 29) with good correlation (R-2 = 0.81) and agreement (Bland-Altman) compared to controls. In combination with previous applications, this point of care platform can potentially detect cell and protein biomarkers simultaneously for sepsis stratification.

• 出版日期2018-5-21